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our experimental results on the crack patterns of shape anisotropic (hard) colloidal particle assembly may further enhance the understanding of the crack patterns in dried suspensions of biological fluids such as blood containing anisotropic soft colloidal particles.

our observations show that with increasing the hematite particle concentration from 0.75 to 3.0wt% (v&/=4.0&/=7.5cm), the pattern of cracks in the dried film change from radial to circular. the crack patterns are illustrated in fig. 2. the observed crack patterns are very similar to that reported in past for spherical particle dispersions 14 and ellipsoidal particles 16 . the order of cracks for both spherical and ellipsoidal particles is same. the cracks are observed to be oriented radially in a circular pattern. the spacing between the cracks is also the same for both particle shapes. the difference in the pattern is in the order of the crack. in both the cases, the radial cracks are observed to be on the exterior edges of the circle. in the case of ellipsoidal particles, the cracks are observed to be ordered in clockwise direction (as seen from the top).

the mechanism to produce cracks is demonstrated in fig. 3. the mechanism is explained in two stages: (i) the formation of water film on the substrate as a result of the capillary effect (ii) the crack formation in the dried film as a result of particle packing.

in the first stage, the water film is formed when the colloid drops are placed on a horizontal surface. the capillary effect on the curved substrate starts the coalescence of colloids and gives rise to a thin layer of colloidal particles on the substrate. the thin layer of colloids gets thicker as the drop grows due to the evaporative process and its size remains constant till the complete evaporation of the water, which is the second stage. during the second stage, the film height increases as the evaporation proceeds. initially, the colloidal film is supported by the substrate but as the film height increases, the film starts to get weak and unsupported. as a result, a crack starts to form in the film. the crack pattern is determined by the size of particles and the spacing between the particles. the crack in the dried film indicates the presence of cracks in the colloidal film. in our experiments the particle size is larger than the thickness of the thin film. the cracks in the dried film are mostly disordered. this is because the cracks are formed when the film is not supported by the substrate as the particle size increases. after the cracking occurs, the particles are deposited in the cracks and the cracks are closed. the drying rate is determined by the evaporation of the colloid and in our experiments, the drying rate is much faster than the timescale of the experiments. due to the fast drying rate, the cracks are observed to be disordered. as the drying is slow and the particles are not deposited in the cracks, the cracks are observed to be ordered. the drying rate is faster for spherical particles and slow for ellipsoidal particles. this results in the formation of cracks with a circular pattern for spherical particles and a radial pattern for ellipsoidal particles.

our experimental results on the crack patterns of shape anisotropic (hard) colloidal particle assembly may further enhance the understanding of the crack patterns in dried suspensions of biological fluids such as blood containing anisotropic soft colloidal particles.
our observations show that with increasing the hematite particle concentration from 0.75 to 3.0wt% (v&/=4.0&/=7.5cm), the pattern of cracks in the dried film change from radial to circular. the crack patterns are illustrated in fig. 2. the observed crack patterns are very similar to that reported in past for spherical particle dispersions 14 and ellipsoidal particles 16 . the order of cracks for both spherical and ellipsoidal particles is same. the cracks are observed to be oriented radially in a circular pattern. the spacing between the cracks is also the same for both particle shapes. the difference in the pattern is in the order of the crack. in both the cases, the radial cracks are observed to be on the exterior edges of the circle. in the case of ellipsoidal particles, the cracks are observed to be ordered in clockwise direction (as seen from the top).
the mechanism to produce cracks is demonstrated in fig. 3. the mechanism is explained in two stages: (i) the formation of water film on the substrate as a result of the capillary effect (ii) the crack formation in the dried film as a result of particle packing.
in the first stage, the water film is formed when the colloid drops are placed on a horizontal surface. the capillary effect on the curved substrate starts the coalescence of colloids and gives rise to a thin layer of colloidal particles on the substrate. the thin layer of colloids gets thicker as the drop grows due to the evaporative process and its size remains constant till the complete evaporation of the water, which is the second stage. during the second stage, the film height increases as the evaporation proceeds. initially, the colloidal film is supported by the substrate but as the film height increases, the film starts to get weak and unsupported. as a result, a crack starts to form in the film. the crack pattern is determined by the size of particles and the spacing between the particles. the crack in the dried film indicates the presence of cracks in the colloidal film. in our experiments the particle size is larger than the thickness of the thin film. the cracks in the dried film are mostly disordered. this is because the cracks are formed when the film is not supported by the substrate as the particle size increases. after the cracking occurs, the particles are deposited in the cracks and the cracks are closed. the drying rate is determined by the evaporation of the colloid and in our experiments, the drying rate is much faster than the timescale of the experiments. due to the fast drying rate, the cracks are observed to be disordered. as the drying is slow and the particles are not deposited in the cracks, the cracks are observed to be ordered. the drying rate is faster for spherical particles and slow for ellipsoidal particles. this results in the formation of cracks with a circular pattern for spherical particles and a radial pattern for ellipsoidal particles.
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